Characterization of the Role of CG12479 in
Drosophila melanogaster Mitochondrial Morphogensis
In Drosophila melanogaster, the fruit fly, CG12479 codes for Mic10, a subunit of the Mitochondrial Contact Site and Cristae Organizing System (MICOS) complex. Localization of the MICOS complex, as well as oligomers of Mic10, on the inner mitochondrial membrane is necessary for mitochondrial cristae formation. Previously conducted studies have shown that mutations in functionally important subunits of the MICOS complex result in abnormal nebenkern morphology. Additionally, phase contrast micrographs of RNAi knocked-down MICOS complex subunits revealed a higher than normal occurrence of dual-lobed nebenkern. This was especially true when Mic10 was knocked down. As a result, this study aimed to determine the specific role of Mic10, in mitochondrial morphology during Drosophila melanogaster spermatogenesis. Phase-contrast imaging of testis from CRISPR mutagenized flies showed abnormally high occurrences of dual-lobed nebenkern as compared to dissections of wild-type flies, and fertility testing of suspected mutants showed a decrease in fertility in some strains. Sequencing of isolated DNA from the suspected CG12479 mutants will confirm the role of CG12479 in the observed phenomenon.